论文部分内容阅读
目的探测进入抗氯喹疟原虫中的氯喹量较敏感株低的原因是受阻于感染的红细胞，还是疟原虫自身，以及抗氯喹疟原虫是否具有快速排出氯喹的特点。方法：给两株感染小鼠ｉｇ氯喹，用高压液相（外标法）检测感染红细胞和疟原虫中的氯喹量。结果：ｉｇ氯喹４．０６ｍｇ／ｋｇ与４００ｍｇ／ｋｇ氯喹３ｈ后，敏感株感染红细胞和抗氯喹株感染红细胞中的氯喹量无明显差异，而抗氯喹疟原虫中的氯喹量，两个剂量组分别较敏感的低５４．０％与４２．１％（Ｐ＜０．００１）。比较ｉｇ氯喹３ｈ与７ｈ后疟原虫积聚的氯喹量，敏感株两者相同，抗性株７ｈ后不仅无明显减少，相反地上升（０．４５２±０．０７９ｎｍｏｌ／ｍｇ蛋白与０．５５９±０．１２４ｎｍｏｌ／ｍｇ）蛋白（Ｐ＜０．０２）。结论：进入抗氯喹疟原虫中的氯喹量低，其受阻部位是在疟原虫本身而不是在感染的红细胞，未发现抗氯喹疟原虫快速排出氯喹。
Objective To investigate whether the lower concentration of chloroquine into chloroquine resistant to chloroquine resistant Plasmodium is dependent on the infected erythrocytes, the Plasmodium itself and whether chloroquine resistant to chloroquine is rapidly excreted. Methods: Two strains of chloroquine were infected in mice and the amount of chloroquine in infected erythrocytes and plasmodium was detected by HPLC (external standard method). Results: After chloroquine 4.06mg / kg and 400mg / kg chloroquine 3h, there was no significant difference in the amount of chloroquine in erythrocytes infected with erythrocytes and chloroquine resistant strains, while the two doses of chloroquine Sensitive lower 54.0% and 42.1% (P <0.001). Comparing the amount of chloroquine accumulating in chloroquine 3h and 7h after chloroquine 3h, the sensitive strains were the same. The resistant strains not only did not decrease obviously but rose on the contrary (0.452 ± 0.079nmol / mg protein and 0.559 ± 0 .124 nmol / mg) protein (P <0.02). CONCLUSIONS: The level of chloroquine entering Plasmodium citrulus is low and the site of inhibition is in the Plasmodium itself but not in the infected erythrocytes. No rapid resistance to chloroquine was found in Plasmodium ovale.