用Ｙ－Ｚ型多功能摇瓶，考察了固定化酵母转化腺苷生产三磷腺苷（ＡＴＰ）过程中ＣＯ２释放率（ＣＥＲ）与葡萄糖、腺苷、腺苷二磷酸、磷酸腺苷在反应液中的积累情况。结果表明，ＣＥＲ在整个反应过程中可分为缓慢上升、快速上升和下降３阶段。根据ＣＥＲ的变化可很快估算出葡萄糖的消耗状况，分析判断出反应进程，便于反应过程的在线控制 Y-Z type multi-purpose shake flask was used to study the effects of glucose, adenosine, adenosine diphosphate and adenosine monophosphate on the reaction of CO2 release rate (CER) during the production of adenosine by immobilized yeast adenosine The accumulation of liquid. The results show that CER can be divided into three stages: slow rise, rapid rise and fall during the whole reaction. According to the changes of CER, the consumption of glucose can be quickly estimated, and the reaction process can be analyzed to facilitate the online control of the reaction process