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目的:探究福州市麻疹病毒基因的遗传变异特点及种系进化分布,为控制和消除麻疹提供科学依据。方法:应用Vero/slam细胞进行麻疹病毒分离,用RT-PCR方法扩增分离株病毒的N基因C末端450个核苷酸片段,扩增产物经纯化后,进行N基因核苷酸序列测定并推导出其aa序列,然后用DNASTAR MegAlign序列分析软件进行基因种系进化特性分析。结果:福州市麻疹分离株均为H1a基因亚型,3株病毒核苷酸同源性为99.3%～100%,在遗传树图中分属于1个独立分支,与疫苗株S191相比核苷酸同源性为87.5%,aa同源性为91.9%～92.1%。结论:福州麻疹野病毒与疫苗株S191在核苷酸与aa序列均存在较大差异,在麻疹监测工作中应进一步加强麻疹病原学的监测和麻疹遗传变异特性的研究。
Objective: To explore the genetic variation and germline evolution of measles virus in Fuzhou and to provide a scientific basis for controlling and eliminating measles. Methods: The virus was isolated from Vero / slam cells. The C-terminal 450 nucleotide fragment of the N gene of the isolated virus was amplified by RT-PCR. The amplified product was purified and the nucleotide sequence of N gene was determined. The aa sequence was deduced, then the DNASTAR MegAlign sequence analysis software was used to analyze the evolutionary characteristics of the germline. Results: All the isolates of measles in Fuzhou were subtype H1a. The nucleotide homology of the three isolates was 99.3% -100%, belonging to one independent branch in the genetic tree map. Compared with the vaccine strain S191, Acid homology was 87.5%, aa homology was 91.9% ~ 92.1%. Conclusion: There is a great difference between nucleotide and aa sequence of wild-type measles virus and vaccine strain S191 in Fuzhou, and monitoring of measles etiology and genetic variation of measles should be further strengthened in the monitoring of measles.