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[目的]探讨水杨酸钠(sodium salicylate,NaSAL)对抗百草枯(paraquat,PQ)致肺损伤的作用机制。[方法]将72只雄性SD大鼠随机分为溶剂对照组(生理盐水灌胃)、PQ染毒组和PQ+NaSAL干预组。PQ经口染毒,剂量为80 mg/kg(按大鼠体重),干预组给予染毒组相等剂量PQ后立即腹腔注射NaSAL(120 mg/kg)。在染毒后第1、3、7、14天取对照组、染毒组、干预组各6只动物进行检查,分别用凝胶电泳迁移率实验(EMSA)和酶联免疫吸附法(ELISA)检测肺组织中核转录因子(NF-κB)活性变增高;肺组织中TNF-α、TGF-β1蛋白表达增强,各时点蛋白水平与对照组相比差异有统计学意义(P<0.05)。干预组NF-κB活性较染毒组降低;与染毒组相比,干预组肺组织TNF-α、TGF-β1蛋白水平表达明显下降,相应时点差异也有统计学意义(P<0.05)。[结论]NaSAL干预后可以降低肺组织中NF-κB的活性,减少TNF-α、TGF-β1的蛋化及肿瘤坏死因子(TNF-α)、转化生长因子-β(1TGF-β1)蛋白含量的改变。[结果]染毒组大鼠肺组织中NF-κB活性白表达,提示NaSAL可以改善PQ中毒引起的肺损伤。
[Objective] To investigate the mechanism of sodium salicylate (NaSAL) against lung injury induced by paraquat (PQ). [Methods] Seventy-two male Sprague-Dawley rats were randomly divided into solvent control group (saline), PQ-treated group and PQ + NaSAL-treated group. PQ was orally exposed to a dose of 80 mg / kg (body weight of rats), and NaSAL (120 mg / kg) was intraperitoneally injected into the intervention group at the same dose of PQ. The control group, the exposure group and the intervention group of 6 animals were inspected on the 1st, 3rd, 7th and 14th day after exposure respectively by EMSA and ELISA, The activity of nuclear factor kappa B (NF-κB) in the lung tissue was increased. The expression of TNF-α and TGF-β1 protein in the lung tissue was increased. The protein level at each time point was significantly different from that in the control group (P <0.05). The activity of NF-κB in the intervention group was lower than that in the control group. Compared with the control group, the expressions of TNF-α and TGF-β1 in the intervention group were significantly decreased (P <0.05). [Conclusion] NaSAL can decrease the activity of NF-κB in lung tissue and decrease the protein expression of tumor necrosis factor (TNF-α), transforming growth factor-β (1TGF-β1) Change. [Results] The expression of NF-κB in lung tissue of rats exposed to NaSAL could improve the lung injury caused by PQ poisoning.