本文介绍了水样中超微量多环芳烃的高效液相色谱—荧光分光光度测定法。工作中选定的分离、检测条件,满足了快速定量的要求,10分钟内可实现七种多环芳烃的分离。定性工作中,除经典的保留值定性之外,还介绍了停流扫描荧光光谱、不同检测条件下的峰高比值,以及同步荧光光谱定性。在定量分析中,此法的最小检出浓度和回收率分别为:蒽0.54ppt(84.4%),荧蒽0.40ppt(100.9%),芘1.10ppt(96.5%),苯并[a]蒽0.03ppt(96.0%),苯并[a]芘0.02ppt(95.1%)。方法的精度及定量线性良好,线性范围达10~3。对实际水样的检验表明。本方法适合水中超微量多环芳烃的定性、定量分析。 This article describes the ultra-trace polycyclic aromatic hydrocarbons in water samples by high performance liquid chromatography - fluorescence spectrophotometry. Selected work in the separation, testing conditions to meet the rapid quantitative requirements, within 10 minutes to achieve the separation of seven PAHs. Qualitative work, in addition to the classic nature of the retention value, but also introduced the stop flow scanning fluorescence spectra, peak height ratio under different detection conditions, and synchronous fluorescence spectroscopy qualitative. In the quantitative analysis, the minimum detectable concentration and recovery of this method were as follows: anthracene 0.54 ppt (84.4%), fluoranthene 0.40 ppt (100.9%), pyrene 1.10 ppt (96.5%), benzo [a] anthracene 0.03 ppt (96.0%), benzo [a] pyrene 0.02ppt (95.1%). The method has good linearity and good linearity. The linear range is 10 ~ 3. Inspection of actual water samples shows. The method is suitable for the qualitative and quantitative analysis of ultra-trace polycyclic aromatic hydrocarbons in water.