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目的探讨Notch1在胃肠道类癌中的表达及其与淋巴结转移和患者预后的关系。方法随机选取东莞市人民医院2005年1月至2007年6月诊治的胃肠道恶性肿瘤75例,其中类癌45例,腺癌30例,免疫组化Envision二步法检查Notch1在肿瘤中的表达,并分析其与类癌患者预后和临床病理参数的关系。结果 Notch1在胃肠道类癌中低表达,阳性率为33.33%,而在腺癌组织中高表达,阳性率(62.22%,28/45),两组比较差异有统计学意义(P<0.05),Notch1在不同临床分期和分化程度患者中存在差异性表达(P<0.05),其阳性率与患者年龄、性别、肿物大小、肿物部位及淋巴结转移等无关(P>0.05)。Kaplan-Meier生存分析显示Notch1表达阳性患者具有更长的生存期(P<0.05)。结论 Notch1在胃肠道类癌中低表达,与肿物分化程度、患者临床分期和预后相关,具有一定的临床意义。
Objective To investigate the expression of Notch1 in gastrointestinal carcinoid and its relationship with lymph node metastasis and prognosis. Methods 75 cases of gastrointestinal cancer diagnosed and treated in Dongguan People’s Hospital from January 2005 to June 2007 were randomly selected, including 45 carcinoid and 30 adenocarcinoma. The immunohistochemical Envision two-step method was used to examine the expression of Notch1 in the tumor Expression, and analysis of its relationship with prognosis and clinicopathological parameters of carcinoid patients. Results The positive rate of Notch1 in gastrointestinal carcinoid was 33.33%, while it was high in adenocarcinoma (62.22%, 28/45). There was significant difference between the two groups (P <0.05) The expression of Notch1 was significantly different between patients with different clinical stage and degree of differentiation (P <0.05). The positive rate of Notch1 was not related to age, gender, tumor size, tumor location and lymph node metastasis (P> 0.05). Kaplan-Meier survival analysis showed that patients with positive Notch1 expression had longer survival (P <0.05). Conclusion The low expression of Notch1 in gastrointestinal carcinoid tumors is related to the degree of tumor differentiation, clinical stage and prognosis of patients, and has certain clinical significance.