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用植物病毒防治剂(TheVirocideforPlant)—TS制剂喷洒感病桑树植株后,用聚丙烯酰胺凝胶电泳对叶片多酚氧化酶、过氧化物酶进行同功酶比较,发现处理株多酚氧化酶的酶带数和对照株相同,均为4条,但酶带位置、强弱有差异;处理株过氧化物酶有4条基本酶带,而对照株有3条酶带,处理株出现一条新酶带.处理株多酚氧化酶活性感病后期比感病初期提高1.6倍,对照株酶活性提高1倍,而过氧化物酶活性感病后期比感病初期下降60%,而对照株酶活性下降80%.用紫外分光光度计测蛋白质含量,处理组为0.3g/L,对照组为0.38g/L,表明经TS制剂处理比对照桑萎缩病的病株蛋白质含量降低.
After spraying the infected mulberry plants with the plant virus controlling agent (TheVirocide for Plant) -TS, the polyphenol oxidase and peroxidase of the leaves were compared by isoenzyme by polyacrylamide gel electrophoresis, and it was found that the polyphenol oxidase The number of enzyme bands was the same as that of the control strains, but the number of bands was 4, but there were differences in the position and intensity of the enzyme bands. There were 4 basic bands in the treated strains and 3 bands in the control strains, Enzyme band. The polyphenol oxidase activity of the treated plants increased by 1.6-fold in the early stages of susceptibility and that of the control plants increased by 1 time, while that of the peroxidase activity decreased by 60% Down 80%. The content of protein in the treatment group was 0.3g / L and the control group was 0.38g / L by UV spectrophotometer, which indicated that the protein content of the diseased plants treated with TS preparation was lower than that of the control.