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目的 :观察小鼠肝癌H2 2 细胞LDLR活性。方法 :对小鼠肝癌H2 2 细胞和正常肝细胞进行受体结合的Scatchard分析和单点分析。结果 :①H2 2 细胞对LDL的亲和性与正常肝细胞相同 ,但H2 2 细胞Bmax明显增多 ;②H2 2 细胞对LDL的非特异性结合、内移和降解能力与正常肝细胞相同。结论 :小鼠肝癌H2 2 细胞LDLR活性增高 ,其对LDL的内移和降解功能未变。
Objective: To observe the LDLR activity of mouse hepatoma H2 2 cells. METHODS: Scatchard analysis and single point analysis of receptor binding in mouse hepatoma H2 2 cells and normal liver cells were performed. Results: ①H2 2 cells had the same affinity to LDL as the normal hepatocytes, but the Bmax of H2 2 cells increased obviously. ② The nonspecific binding, internal migration and degradation of H2L 2 cells to LDL were the same as normal hepatocytes. Conclusion: The LDLR activity of mouse hepatocarcinoma H2 2 cells is increased, and the function of LDL migration and degradation is unchanged.