利用苯乙酸与捕捉剂反应生成的沉淀 ,确定固定化青霉素G酰化酶的催化活性部位 .对不同酶活的固定化青霉素G酰化酶进行了活性定位的研究 ,发现酶活的部位集中分布在载体的表面上 ,且分布不均匀 ,而断面上几乎没有酶活存在 ,证明了该方法的可靠性与准确性 The precipitation of immobilized penicillin G acylase was determined by the precipitation reaction of phenylacetic acid and trapping agent.It was found that the active sites of immobilized penicillin G acylase with different activities were concentratedly distributed On the surface of the carrier, and the distribution is not uniform, but almost no enzyme activity exists on the cross section, which proves the reliability and accuracy of the method