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对1655份普通野生稻(Oryza rufipogon Griff.)和31份药用野生稻(O.officinalis Wall.ex Watt)进行了细菌性条斑病(Xanthomonas oryzae pv.oryzicola,简称细条病)抗性鉴定,结果发现在普通野生稻中有57份抗病材料,其中3级抗性有31份,占总数的1.87%;5级中抗有26份,占总数1.57%。在药用野生稻资源中,有15份抗病材料,占总数的48.4%。选取了8份普通野生稻抗性资源(分别命名为DP1、DP3、DP5、DP9、DP15、DP16、DP17和DP20)与9311杂交,再自交或与9311回交后,分别获得BC1、F1和F2后代。在接种鉴定中发现这些抗性资源的BC1或F1所有植株均对细条病表现感病,说明这8份材料的抗性属于隐性遗传。在DP3与9311杂交的F2群体中,抗感植株的分离比符合1∶15的比例,说明DP3的抗性由2对隐性重叠作用基因控制。研究结果表明,在野生稻中可以获得一批具有较大利用价值的细条病抗性资源,其中药用野生稻资源中抗性材料所占的比例较大。
Resistance to bacterial leaf streak (Xanthomonas oryzae pv.oryzicola) was evaluated on 1655 Oryza rufipogon Griff. And 31 O. officinalis Wall. Ex Watt. The results showed that there were 57 disease-resistant materials in common wild rice, of which 31 were Grade 3 resistance, accounting for 1.87% of the total; 26 were resistant to Grade 5, accounting for 1.57% of the total. Among medicinal wild rice resources, there are 15 disease-resistant materials, accounting for 48.4% of the total. Eight common wild rice resistance resources named DP1, DP3, DP5, DP9, DP15, DP16, DP17 and DP20 were selected and crossed with 9311. After selfing or backcrossing with 9311, BC1, F1 and F2 offspring. All the plants of BC1 or F1 which found these resistance resources in inoculation identification showed susceptibility to the disease, which indicated that the resistance of the 8 materials belonged to recessive inheritance. In F2 populations of DP3 and 9311 crossed, the segregation ratio of anti-susceptible plants was in the ratio of 1:15, indicating that DP3 resistance was controlled by two pairs of recessive overlapping genes. The results showed that a number of stripe virus resistance resources with great utilization value could be obtained in wild rice, of which the proportion of resistant materials in medicinal wild rice resources was larger.