【摘 要】
：
目的 快速查明2021年8月芜湖市某社区卫生服务中心医务人员咽拭子标本SARS-CoV-2核酸阳性原因,提升COVID-19精准防控能力.方法 应用实时荧光RT-PCR技术,对某社区卫生服务中心医务人员10∶1咽拭子混采管标本(某第三方检测机构SARS-CoV-2核酸初筛阳性)及重新采集的咽拭子单采管标本进行SARS-CoV-2核酸复核检测,核酸阳性标本进行SARS-CoV-2 D614G突变位点检测.随后采集该社区卫生服务中心外环境涂抹样进行SARS-CoV-2核酸污染状况评估检测.结果 3份10∶1咽
【机 构】
：
芜湖市疾病预防控制中心,安徽241000
论文部分内容阅读
目的 快速查明2021年8月芜湖市某社区卫生服务中心医务人员咽拭子标本SARS-CoV-2核酸阳性原因,提升COVID-19精准防控能力.方法 应用实时荧光RT-PCR技术,对某社区卫生服务中心医务人员10∶1咽拭子混采管标本(某第三方检测机构SARS-CoV-2核酸初筛阳性)及重新采集的咽拭子单采管标本进行SARS-CoV-2核酸复核检测,核酸阳性标本进行SARS-CoV-2 D614G突变位点检测.随后采集该社区卫生服务中心外环境涂抹样进行SARS-CoV-2核酸污染状况评估检测.结果 3份10∶1咽拭子混采管标本中2份核酸双靶标(ORF1ab+N)阳性,35份医务人员和医学实习生咽拭子单采管标本核酸双靶标阴性.混采管阳性标本未发生SARS-CoV-2 D614G位点变异,推断来源于SARS-CoV-2野生株(疫苗株).12份外环境标本中3份单靶标阳性,其中1份ORF1ab基因阳性,来源于COVID-19疫苗接种人员工作服;2份N基因阳性,来源于COVID-19疫苗接种室键盘和普通疫苗接种室键盘鼠标.结论 某社区卫生服务中心医务人员咽拭子混采管标本SARS-CoV-2核酸阳性是由COVID-19疫苗污染导致.
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