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目的探讨妊娠糖尿病大鼠视黄醇结合蛋白4(RBP-4)水平的变化及与胰岛素抵抗的关系。方法将20只健康SD孕鼠随机分为妊娠糖尿病大鼠(GDM)组10只、正常妊娠对照组(NC)10只。采用链脲佐菌素(STZ)腹腔一次性注射制备GDM大鼠模型。各组大鼠于分娩前及妊娠20d,测定空腹血糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、空腹胰岛素水平(FINS)和血清RBP-4水平,计算胰岛素抵抗指数(HOMA-IR)。结果①GDM组大鼠血清FPG、FINS、TG、TC、LDL-C、RBP-4、HOMA-IR高于正常妊娠对照组(P<0.05,P<0.01);②Pearson相关分析显示,GDM组大鼠HOMA-IR与FPG、FINS、TG、TC、LDL-C、RBP-4呈正相关(P<0.05,P<0.01)。③多元线性逐步回归分析结果提示,TG、RBP-4是影响GDM组大鼠HOMA-IR独立的危险因素。结论 RBP-4水平的升高与GDM胰岛素抵抗有关。
Objective To investigate the changes of retinol binding protein 4 (RBP-4) in gestational diabetes mellitus (DM) rats and its relationship with insulin resistance. Methods Twenty pregnant SD rats were randomly divided into 10 pregnant rats with gestational diabetes mellitus (GDM) and 10 normal pregnant controls (NC). GDM rat model was established by intraperitoneal injection of streptozotocin (STZ). The levels of fasting blood glucose (FPG), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), fasting insulin (FINS) and serum RBP-4 levels, calculate the insulin resistance index (HOMA-IR). Results ① The levels of FPG, FINS, TG, TC, LDL-C, RBP-4 and HOMA-IR in GDM group were significantly higher than those in normal pregnancy group (P <0.05, HOMA-IR was positively correlated with FPG, FINS, TG, TC, LDL-C and RBP-4 (P <0.05, P <0.01). ③ Multiple linear stepwise regression analysis suggested that TG and RBP-4 were risk factors for HOMA-IR independent of GDM rats. Conclusion The increase of RBP-4 level is related to the insulin resistance of GDM.