论文部分内容阅读
在血液中测定土霉素的含量是检查土霉素片剂在动物体内生物利用度的一个重要课题。在生物样品中测定土霉素的常用方法有微生物法和化学方法。微生物法灵敏度较高,但费时间及选择性不好。化学方法中的极谱法及比色法灵敏度均不高,而萤光光度法则灵敏度较高及操作比较简便。在文献中报导的萤光法有:Hayes等将土霉素在酸中加热转变成脱氢衍生物,在硷性中测定萤光强度。Ibsen等利用镁离子与土霉素所形成的整合物测定萤光。Kohn利用钙离子及巴比土酸盐与土霉素形成络合物而测定萤光。1976年Poiger等在Kohn的研究基础上提出在生物样品中用萤光光度法测定四环素族药物的方法。本文主要是参考Poiger的方法来测定狗在口服土霉素片后血液中土霉素的浓度。
Determination of oxytetracycline content in the blood is to check oxytetracycline tablet bioavailability in animals is an important issue. Common methods for the determination of oxytetracycline in biological samples include microbial and chemical methods. Microbiological sensitivity higher, but time-consuming and poor selectivity. Polarography chemistry and colorimetric sensitivity are not high, while the fluorescence luminosity law of high sensitivity and the operation is relatively simple. Fluorescence methods reported in the literature include Hayes et al., Which converts oxytetracycline into a dehydrogenated derivative by heating in acid and measuring the fluorescence intensity in alkaline. Ibsen and other use of magnesium ions and oxytetracycline formed complexes formed fluorescence. Kohn measured fluorescence using calcium ions and barbiturates to form complexes with oxytetracycline. In 1976, Poiger et al. Proposed a method for the determination of tetracycline drugs by fluorescence spectrophotometry in biological samples based on Kohn’s research. This paper mainly refers to Poiger’s method to determine oxytetracycline oxytetracycline oxytetracycline tablets in dogs after oxytetracycline concentration.