检测肿瘤坏死因子(TNF)活性方法以染料摄入法易被人们接受,因该法一次可做多组分检测,无主观因素、简便快速。但其弊端是只能用贴壁生长较牢固的肿瘤细胞为靶细胞。对悬浮生长和贴壁不牢的肿瘤细胞不适用。本文采用甲基纤维素半固体培养法以悬浮生长和贴壁不牢的肿瘤细胞为靶细胞,检测大鼠血清TNF(以下简称RTNS)活性。 Detection of tumor necrosis factor (TNF) activity by dye intake easily accepted by the people, because the method can be multi-component test, no subjective factors, simple and quick. But its drawback is that only adherent growth of solid tumor cells as target cells. Not suitable for suspension growth and adherent tumor cells. In this paper, methylcellulose semi-solid culture method to suspension growth and adherent tumor cells as target cells, the detection of serum TNF (hereinafter referred to as RTNS) activity.