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目的通过对去势大鼠加载不同时段的低载荷机械振动,探讨不同的振动持续时间对骨质疏松大鼠骨组织成骨相关蛋白表达的影响。方法 6个月龄雌性Wistar大鼠24只,随机分为8周去势对照组(C8)、12周去势对照组(C12)、8周振动组(V8)和12周振动组(V12)。所有大鼠接受去势手术1周后开始实施振动试验,对V8组与V12组大鼠分别加载35 Hz、0.25 g的低载荷机械振动,每天15 min;去势对照组不做处理。8周与12周后分两批断颈处死大鼠,采用Western blot技术检测各组大鼠成骨相关蛋白BMP-2、p-ERK、Runx2、OCN的表达。结果低载荷机械振动能够显著上调去势大鼠骨组织BMP-2、p-ERK、Runx2和OCN的蛋白表达水平(P<0.01),随着振动时间的延长,V12组较V8组蛋白含量分别增加了22.61%(P<0.05)、27.96%(P<0.01)、25.85%(P<0.01)、27.05%(P<0.01);去势对照组间各蛋白表达水平无明显差异(P>0.05)。结论低载荷机械振动能够上调骨组织成骨相关蛋白的表达水平,并随着振动时间的增加显现出更为优越的成骨作用。
OBJECTIVE: To investigate the effect of different duration of vibration on the osteogenesis-related protein expression in osteoporosis rats by applying low-load mechanical vibration at different time points in ovariectomized rats. Methods Twenty-four female Wistar rats of 6 months old were randomly divided into 8 weeks castration control group (C8), 12 weeks castration control group (C12), 8 weeks vibration group (V8) and 12 weeks vibration group (V12) . Vibration test was carried out in all rats one week after castration surgery. V8 and V12 rats were respectively loaded with low-load mechanical vibration of 35 Hz and 0.25 g at 15 min every day. The castration control group was not treated. The rats were sacrificed at two weeks and eight weeks after fracture, and the expression of osteogenesis-related protein BMP-2, p-ERK, Runx2 and OCN were detected by Western blot. Results Low-load mechanical vibration could significantly upregulate BMP-2, p-ERK, Runx2 and OCN protein expression in ovariectomized rats (P <0.01). With the increase of vibration time, Increased 22.61%, 27.96% (P <0.01), 25.85% (P <0.01), and 27.05% (P <0.01) respectively. There was no significant difference in the expression of all the proteins among castration control groups (P> 0.05 ). Conclusion Low-load mechanical vibration can up-regulate the expression of osteogenesis-related proteins in bone tissue and show more superior osteogenesis with the increase of vibration time.