论文部分内容阅读
试验证明,直接荧光诊断法是诊断柑桔黄龙病的一种快速、简便、准确的方法。 将健叶和黄龙病叶叶柄用保险刀片徒手切片,在透射式荧光显微镜下检查,可看到健叶叶柄的木质部导管细胞壁发黄色荧光,韧皮纤维细胞发绿色荧光,而病叶叶柄韧皮部中有1～多个鲜明的黄色或黄绿色荧光团块。这种特异性的荧光,在健叶柄韧皮部中是没有的,其它由病毒、类病毒、细菌、真菌等病原引起的病叶柄韧皮部中亦没有此种特异性荧光。
Experiments show that direct fluorescence diagnosis is a rapid, simple and accurate method for the diagnosis of citrus Huanglongbing. The leaves of healthy and yellow dragon leaves were manually sliced with an insurance blade and examined under a transmission fluorescence microscope to see that the xylem ductal cell wall of the healthy leaf petiole fluoresced yellow and the phloem fiber cells fluoresced green whereas in the diseased leaf petiole phloem There are 1 or more distinct yellow or yellow-green fluorophores. This specific fluorescence was absent in the phloem of the healthy petiole and was not found in the phloem of other petioles caused by viruses, viruses, bacteria, fungi and other pathogens.