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目的:研究氨基酸和脂类在胃癌组织和血浆中的代谢变化,揭示胃癌组织和血浆之间的代谢联系。方法:应用基于气相色谱-飞行时间质谱(GC/TOFMS)的测定技术,检测20份慢性胃炎组织、17份胃癌组织、15份慢性胃炎患者血浆、15份胃癌患者血浆和15份胃癌术后患者血浆中的氨基酸和脂类的水平,结合t检验分析数据。结果:胃癌组织内氨基酸水平较慢性胃炎组织升高或相当,尤以半胱氨酸(P=0.007)、2-氨基己二酸(P=0.045)、谷氨酰胺(P=0.047)明显;胃癌患者血浆中的氨基酸水平低于慢性胃炎患者血浆,尤其半胱氨酸(P=0.029)、丝氨酸(P=0.013)、甘氨酸(P=0.043)。与慢性胃炎组织相比,胃癌组织中3-羟基丁酸(P=0.003)、十六碳烯酸(P=0.030)、庚酸(P=0.047)明显增高,胆固醇(P=0.003)明显降低。胃癌术后患者血浆中氨基酸和脂类的水平向慢性胃炎状态恢复。结论:本研究揭示了胃癌组织中氨基酸和脂类代谢异常影响了血浆中的氨基酸和脂类;基于GC/TOFMS的测定方法是研究胃癌代谢的合适平台。
Objective: To study the metabolic changes of amino acids and lipids in gastric cancer tissues and plasma and to reveal the metabolic linkages between gastric cancer tissues and plasma. Methods: Plasma samples from 20 patients with chronic gastritis, 17 patients with gastric cancer, 15 patients with chronic gastritis, 15 patients with gastric cancer and 15 patients with postoperative gastric cancer were detected by gas chromatography-time of flight mass spectrometry (GC / TOFMS) Plasma levels of amino acids and lipids, combined with t-test analysis of the data. Results: The levels of amino acids in gastric cancer tissue were higher than those in chronic gastritis tissues, especially cysteine (P = 0.007), 2 - aminoadipic acid (P = 0.045) and glutamine (P = 0.047) The plasma levels of amino acids in patients with gastric cancer were lower than those in patients with chronic gastritis, especially cysteine (P = 0.029), serine (P = 0.013) and glycine (P = 0.043). Compared with chronic gastritis, 3-hydroxybutyrate (P = 0.003), palmitoleic acid (P = 0.030) and heptanoic acid (P = 0.047) were significantly increased and cholesterol (P = 0.003) . The level of amino acids and lipids in the plasma of postoperative patients with gastric cancer recovered to chronic gastritis. CONCLUSIONS: This study revealed that abnormal amino acids and lipids in gastric cancer affect plasma amino acids and lipids; GC / TOFMS-based assays are suitable platforms for the study of gastric cancer metabolism.