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目的优选聚酰胺纯化枳实总黄酮的工艺条件。方法采用紫外分光光度法,以枳实总黄酮的吸附率、解吸率作为考核指标,筛选出聚酰胺纯化枳实总黄酮的最佳工艺参数。结果最佳纯化工艺条件:上样液浓度为3.0 mg.mL-1,pH值为6.0～7.0,上样量为1 BV,吸附1.5 h后,先用2 BV的水洗脱,再用3 BV的80%乙醇洗脱。结论聚酰胺树脂对枳实总黄酮的纯化效果好,成本低,可工业化推广。
Objective To optimize the polyamide purification conditions of the total flavonoids. Methods Ultraviolet spectrophotometry was used to determine the optimum process parameters for purification of total flavonoids from Polygonum Immiturae Immaturus with the adsorption and desorption rates of total flavonoids of Fructus Aurantii Immaturus. Results The optimal conditions for the purification were as follows: the concentration of sample solution was 3.0 mg.mL-1, the pH was 6.0-7.0, the sample volume was 1 BV. After 1.5 h of adsorption, BV 80% ethanol. Conclusion Polyamide resin has good purification effect on total flavonoids of Fructus Aurantii Immaturus, with low cost and industrialization.