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背景:脑脊液和血浆中神经元特异性烯醇化酶(NSE)和神经组织蛋白S-100β含量在脑缺血性损伤时升高的程度能反映脑损伤的程度和预后。目的:观察大鼠脑缺血再灌注损伤脑组织NSE和S-100β的变化规律及其意义。设计:随机对照的研究。地点及对象:本实验在青岛大学医学院脑血管病研究所和山东省脑血管病防治重点实验室完成。成年健康雌性SD大鼠36只,体质量230～270g,清洁级,由中国科学院上海实验动物中心提供。方法:实验由作者完成。方法:用线栓法建立大鼠大脑中动脉缺血再灌注模型,应用神经等级评分观察脑缺血再灌注后行为功能的恢复,免疫组织化学法检测脑组织中NSE和S-100β的动态变化。主要观察指标:各组大鼠皮质区、纹状体NSE,S-100β含量及神经功能评分。结果:皮质区、纹状体区的NSE和S-100β免疫阳性反应均于缺血再灌注后12h明显增强,并随时间变化而逐渐下降,至14d降至正常水平。神经功能评分再灌注2h～1d时为3.00分,再灌注3,7,14d恢复至1.50分(t=2.60～4.48,P<0.05)。结论:脑缺血再灌注损伤后脑组织NSE和S-100β蛋白表达增加,并与神经功能恢复有相关性。
BACKGROUND: The levels of neuron-specific enolase (NSE) and neuronal tissue protein S-100β in cerebrospinal fluid and plasma during cerebral ischemic injury reflect the degree and prognosis of brain injury. OBJECTIVE: To observe the changes of NSE and S-100β in brain tissue after cerebral ischemia-reperfusion injury in rats and its significance. Design: A Randomized Controlled Study. Location and Subjects: This experiment was performed at the Institute of Cerebrovascular Diseases, Medical College of Qingdao University and Key Laboratory of Prevention and Treatment of Cerebrovascular Diseases in Shandong Province. Thirty-six adult healthy female Sprague-Dawley rats weighing 230-270g were provided with the Shanghai Experimental Animal Center, Chinese Academy of Sciences. Method: The experiment is done by the author. Methods: The model of middle cerebral artery occlusion (MCAO) was established by thread occlusion. The neurological score was used to observe the behavioral recovery after cerebral ischemia-reperfusion. The dynamic changes of NSE and S-100β in brain tissue were detected by immunohistochemistry . MAIN OUTCOME MEASURES: Cortical area, striatum NSE, S-100β content and neurological function scores of rats in each group. Results: The immunoreactivity of NSE and S-100β in cortex and striatum were significantly increased at 12 h after ischemia-reperfusion, and gradually decreased with time, then decreased to normal level on 14 d. The score of neurological score was 3.00 at 2h ~ 1d after reperfusion, and recovered to 1.50 at 3,7,14d after reperfusion (t = 2.60 ~ 4.48, P <0.05). Conclusion: The expression of NSE and S-100β in brain tissue increases after cerebral ischemia-reperfusion injury, and has correlation with neural function recovery.