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用不连续密度梯度离心法分离恶性胸液淋巴细胞（ＭＰＥＬ）及肿瘤细胞，比较了ＭＰＥＬ与外周血淋巴细胞（ＰＢＬ）对自体肿瘤细胞（ＡＴＣ）的杀伤活性。结果３组不同密度梯度中以１００％：６０％的密度比构成的不连续密度梯度分离效果较佳。ＰＢＬ对ＡＴＣ的杀伤活性明显高于ＭＰＥＬ（Ｐ＜０．００１），但经重组白细胞介素２（ｒＩＬ２）激活的ＭＰＥＬ的扩增能力及杀伤活性均较经ｒＩＬ２激活的ＰＢＬ（即ＬＡＫ细胞）明显为高（Ｐ＜０．００１）。表明恶性胸液患者胸腔局部免疫功能呈抑制状态，经ｒＩＬ２激活可得到改善。活化ＭＰＥＬ是较ＬＡＫ更适于肿瘤过继免疫治疗的效应细胞。
Malignant pleural fluid lymphocytes (MPEL) and tumor cells were isolated by discontinuous density gradient centrifugation. The killing activity of MPEL and peripheral blood lymphocytes (PBL) on autologous tumor cells (ATC) was compared. Results Discontinuous density gradient of 100%: 60% of the density gradient of three different density gradients was better. The killing activity of PBL against ATC was significantly higher than that of MPEL (P <0.001). However, the proliferation and cytotoxicity of MPEL activated by recombinant interleukin 2 (rIL2) were higher than that of PBL activated by rIL2 (LAK cells) Significantly higher (P <0.001). Show that the malignant pleural effusion in patients with local immune function was inhibited by rIL2 activation can be improved. Activated MPEL is an effector cell that is more suitable for adoptive immunotherapy of tumors than LAK.