论文部分内容阅读
目的 探讨原癌基因C myc ,H ras蛋白产物P6 2和P2 1在胆管癌、胆囊癌和胆道胆囊良性病变中的表达及其临床意义。方法 应用免疫组织化学方法 (ABC法 ) ,检测 30例胆管癌 ,2 8例胆囊癌和 30例胆道良性病变中P6 2和P2 1的表达水平。结果 30例胆管癌中 ,P6 2和P2 1过度表达阳性率分别为 6 6 .7%和 6 3.3% ,而胆管良性病变阳性率分别为 10 %和 0 ,二者差异有显著性意义 (P <0 .0 5 ) ,2 8例胆囊癌中 ,P6 2和P2 1过度表达阳性率分别为 42 .8%和 5 7.1% ,而胆囊良性病变仅为 10 %和 5 % ,二者差异比较有显著意义 (P <0 .0 5 )。同时也显示 ,胆管和胆囊恶性病变中P6 2和P2 1过度表达程度与病理组织学相一致 ,与原发癌灶的大小 ,淋巴的转移有显著相关性。结论 胆管癌、胆囊癌中P6 2和P2 1水平呈现过度表达 ,与病理类型、恶性程度、癌灶大小及淋巴转移有关 ,对其诊断、鉴别诊断及预后的判断有一定的参考价值。
Objective To investigate the expression and clinical significance of proto-oncogenes C myc and H ras protein products P6 2 and P 2 1 in cholangiocarcinoma, gallbladder carcinoma and benign gallbladder gallbladder lesions. Methods Immunohistochemical method (ABC method) was used to detect the expression of P6 2 and P21 in 30 cases of cholangiocarcinoma, 28 cases of gallbladder carcinoma and 30 cases of benign biliary tract lesions. Results In 30 cases of cholangiocarcinoma, the positive rates of P6 2 and P 2 1 overexpression were 66.7% and 63.3%, respectively, while the positive rates of benign biliary duct lesions were 10% and 0 respectively. There was a significant difference between them (P<0.05). <0. 0 5) The positive rates of P6 2 and P 2 1 overexpression in 42 gallbladder carcinomas were 42.8% and 5 7.1%, respectively, while the gallbladder benign lesions were only 10% and 5% respectively. Significant (P <0. 0 5). It was also shown that the degree of overexpression of P6 2 and P21 in malignant lesions of the bile duct and gallbladder was consistent with pathological histology, and was significantly correlated with the size of primary foci and lymphatic metastasis. Conclusions The levels of P6 2 and P 2 1 in cholangiocarcinoma and gallbladder carcinoma are over-expressed, and are related to pathological types, malignancy, tumor size and lymph node metastasis. It has certain reference value for diagnosis, differential diagnosis and prognosis.