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目的观察重组中国人γ干扰素(INF-γ)对博莱霉素(BLM)所致小鼠肺损伤(PI)的防治作用。方法75只C57小鼠随机分为对照组、BLM组、BLM+INF-γ小剂量组(0.25μg/d)、BLM+INF-γ中剂量组(0.5μg/d)、BLM+INF-γ大剂量组(1.0μg/d),每组15只。实验组小鼠以BLM诱导PI,分别用不同剂量的INF-γ进行防治。对照组小鼠不进行任何处理。实验结束后处死小鼠分别进行肺羟脯氨酸(hydroxyproline,Hyp)测定,用图像分析法计算Ⅰ、Ⅲ型胶原面积与切片面积,肺泡面积与肺视野面积比。结果对照组小鼠肺组织Hyp为(0.65±0.06)μg/mg、BLM组为(0.78±0.08)μg/mg,两者比较差异有统计学意义(P<0.01);对照组Ⅰ、Ⅲ型胶原为(0.004±0.001)、BLM组为(0.048±0.006),两者比较差异有统计学意义(P<0.01);BLM+INF-γ大剂量组大鼠肺组织Hyp为(0.67±0.08)、Ⅰ、Ⅲ型胶原含量为(0.008±0.001)与对照组相比差异均有统计学意义(P均<0.01);各组肺泡与肺组织面积比随着INF-γ的剂量增加而增高,分别为1.78、0.12、0.67、0.73、1.65。结论INF-γ能有效抑制成纤维细胞向肌成纤维细胞转化,减少肺内胶原合成,对PI有一定防治作用。
Objective To observe the preventive and therapeutic effects of recombinant human interferon-gamma (IFN-γ) on lung injury induced by bleomycin (BLM) in mice. Methods 75 C57 mice were randomly divided into control group, BLM group, BLM + INF-γ low dose group (0.25μg / d), BLM + INF-γ medium dose group High-dose group (1.0μg / d), 15 in each group. The mice in the experimental group were induced with BLM by PI, and were respectively treated with different doses of INF-γ. Control mice did not receive any treatment. The mice were sacrificed at the end of the experiment, respectively, hydroxyproline (hydroxyproline, Hyp) determination, image analysis method to calculate type Ⅰ, Ⅲ collagen area and slice area, alveolar area and lung field area ratio. Results Compared with control group, Hyp in lung tissue was (0.65 ± 0.06) μg / mg in BLM group and (0.78 ± 0.08) μg / mg in BLM group, the difference was statistically significant (P <0.01) (P <0.01). The Hyp of lung tissue in BLM + INF-γ high-dose group was (0.67 ± 0.08) (P <0.01). The area ratio of alveolar and lung tissue in each group increased with the increase of the dosage of INF-γ, and the content of collagen typeⅠ and Ⅲ was (0.008 ± 0.001), the difference was statistically significant Respectively, 1.78,0.12,0.67,0.73,1.65. Conclusion INF-γ can effectively inhibit the transformation of fibroblasts to myofibroblasts and reduce the collagen synthesis in the lung, which has a certain preventive and therapeutic effect on PI.