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应用甲醇—水(83∶17)为流动相,在Shim-PackC18柱上,以2,4—二硝基氯苯为内标物,建立了高含量氟氯芳酮的高效液相色谱分析方法。方法的相对标准偏差<0.2%,氟氯芳酮进样量在0.5~5μg内呈良好的线性关系。
The mobile phase was methanol-water (83:17). On the Shim-Pack C18 column, 2,4-dinitrochlorobenzene was used as the internal standard to establish a high performance liquid chromatographic method . The relative standard deviation (RSD) was less than 0.2%, and the linear range of fluconazole injection was 0.5-5 μg.