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应用十二烷基磺酸钠作为疏水离子流动相添加剂，确保氨基酸在流动相中以阳离子形式存在的ｐＨ值条件下，研究了影响氨基酸分离的主要因素，找出了最佳色谱分离条件。
Using sodium dodecyl sulfate as hydrophobic ion mobile phase additive and ensuring that the amino acid exists as a cation in the mobile phase, the main factors influencing the amino acid separation were studied and the optimal chromatographic conditions were found.