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采用ифА法对某些最常见的妇科病患者血液CA-125抗原浓度进行测定分析,目的在于拟定诊断依据和对疗效进行监测。用苏制《CA-125》(?)试验盒,取单克隆抗体包被聚乙烯孔板与含相应抗原的待检血清一起孵育,并加入辣根过氧物酶结合的抗体溶液,孵育过程中生成孔板-抗体+抗原+过氧物酶-抗体复合物。经洗涤、中止反应。显示颜色立刻用酶标光度计测定其光密度。研究对象为有手术指征而住院的108例妇女,患者年龄分布:18～19岁2例,21～30岁42例、31～40岁39例、41～50岁14例和50岁以上者11例。结合
The ифА method was used to determine the concentration of blood CA-125 antigen in some of the most common gynecological patients, with the purpose of formulating the diagnosis basis and monitoring the curative effect. With the Soviet Union “CA-125” (?) Test box, take the monoclonal antibody coated polyethylene plate with the corresponding antigen incubated sera were incubated with horseradish peroxidase-conjugated antibody solution, the incubation process In the resulting plate - antibody + antigen + peroxidase - antibody complex. After washing, stop the reaction. Display color immediately with an enzyme-labeled photometer to measure the optical density. The subjects were hospitalized with surgery indications of 108 women, the age distribution of patients: 18 to 19 years in 2 cases, 21 to 30 years in 42 cases, 31 to 40 years in 39 cases, 41 to 50 years in 14 cases and 50 years of age 11 cases. Combined