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目的探讨乙型肝炎病毒DNA与前S1蛋白及HBcAg在乙肝患者血清中的相关性及临床意义。方法对320例乙肝的血清采用酶联免疫吸附法(ELISA)检测前S1抗原、HBcAg,定量PCR检测HBV-DNA。结果HBV-DNA阳性的140例中HBcAg阳性检出率为84.29%,前Sl抗原的阳性检出率为80.00%,在前S1抗原阳性的99例中HBcAg阳性率为18.18%,在前S1抗原的阴性78例中,HBcAg阳性率为7.69%,两者比较差异有统计学意义(P<0.05)。前S1抗原与HBcAg阳性患者的HBV-DNA拷贝数大部分在103～108IU/ml之间,且HBcAg的阳性率明显高于前S1阳性率,当HBV-DNA在103～106IU/ml时,前S1的阳性率高于HBcAg的阳性率。结论前S1抗原、HBcAg与HBV-DNA符合率较高,检测前S1抗原和HBcAg同样具有重要的临床意义。
Objective To investigate the correlation between hepatitis B virus DNA and serum pre-S1 protein and HBcAg in patients with hepatitis B and its clinical significance. Methods Serum of 320 cases of hepatitis B were detected by enzyme-linked immunosorbent assay (ELISA) pre-S1 antigen, HBcAg, quantitative PCR detection of HBV-DNA. Results The positive rate of HBcAg in the positive cases of HBV-DNA was 84.29%, the positive rate of pre-S1 antigen was 80.00%, the positive rate of HBcAg in pre-S1 antigen-positive 99 cases was 18.18% Negative 78 cases, HBcAg positive rate was 7.69%, the difference between the two was statistically significant (P <0.05). The majority of HBV-DNA copies of pre-S1 antigen and HBcAg-positive patients were between 103 and 108 IU / ml, and the positive rate of HBcAg was significantly higher than that of pre-S1. When HBV-DNA was between 103 and 106 IU / ml, The positive rate of S1 is higher than the positive rate of HBcAg. Conclusion The coincidence rate of pre-S1 antigen, HBcAg and HBV-DNA is high, so it is of great clinical significance to detect pre-S1 antigen and HBcAg.