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本文旨在探讨体脂与骨量的关系,以及调节体脂的瘦素对骨的作用。选用6月龄雌性Wistar大鼠40只,随机分为两组,一组切除双侧卵巢,另一组行假手术。饲养2月后采用ELISA检测血清中瘦素浓度,检测大鼠体质量、腹腔内脂肪含量,DEXA测定大鼠股骨骨密度(BMD)。结果提示大鼠体质量在去卵巢组增加明显(P<0.05),腹腔内脂肪量在去卵巢后增加不明显(P=0.499),脂肪细胞分泌的瘦素两组之间没有差异(P=0.166),去卵巢组单位体质量的骨矿含量(BMC)较假手术组明显降低(P=0.003)。第8周体质量在假手术组与单位体质量BMC负相关,在去卵巢组与BMD正相关,假手术组腹腔内脂肪含量及瘦素浓度与单位体质量的BMC呈负相关关系。因此,体脂、瘦素与单位体质量BMC相关。
This article aims to explore the relationship between body fat and bone mass, and the role of body fat in regulating leptin on bone. Forty female Wistar rats aged 6 months were randomly divided into two groups. One group had bilateral ovaries removed and the other group had sham operation. Serum leptin concentration was detected by ELISA after 2 months of feeding. The body weight, intra-abdominal fat content and the bone mineral density (BMD) of rats were measured by DEXA. The results showed that the body weight of rats increased significantly in the ovariectomized group (P <0.05), the amount of intra-abdominal fat increased slightly after ovariectomy (P = 0.499), but there was no difference between the two groups (P = 0.166). The bone mass per unit body weight (BMC) in the ovariectomized group was significantly lower than that in the sham operation group (P = 0.003). Bone mass was negatively correlated with unit mass in sham operation group and positive correlation with BMD in ovariectomized group. The intraperitoneal fat content and leptin concentration in sham group were negatively correlated with BMC per unit body weight. Therefore, body fat, leptin and body mass BMC related.