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目的探讨外源性透明质酸(HA)是否可以降低巨噬细胞嗜性(CCR5依赖,R5)人类免疫缺陷病毒(HIV)对CD4+T细胞的感染性。方法首先用TZM-bl细胞和未受刺激的CD4+T细胞检测,以评估外源性透明质酸(HA)能否降低R5-HIV的感染性。用透明质酸酶去处理未受刺激的CD4+T细胞,研究内源性HA对R5-HIV感染性的影响。最后,同时测量外源性和内源性透明质酸(HA)对R5-HIV对CD4+T细胞粘和力的影响。结果 (1)100μg外源性HA处理能够显著降低R5-HIV感染TZM-bl细胞和未受刺激的CD4+T细胞(P<0.001)。(2)透明质酸酶处理可增强HIV的感染性,但是如果加上100μg外源性HA可以逆转透明质酸酶的处理(P<0.001)。(3)100μg外源性HA可以减少R5-HIV对CD4+T细胞的粘和力,透明质酸酶处理可以提高R5-HIV对CD4+T细胞的粘和力(P<0.001)。结论外源性HA减少R5-HIV对未受刺激的CD4+T细胞的感染性,而透明质酸酶处理可以提高R5-HIV对CD4+T细胞的粘和力,能增强R5-HIV对CD4+T细胞的感染性。
Objective To investigate whether exogenous hyaluronic acid (HA) can reduce the infectivity of CD4 (superscript +) T cells by macrophage cytotoxicity (CCR5-dependent, R5) human immunodeficiency virus (HIV). Methods TZM-bl cells and unstimulated CD4 + T cells were first used to assess whether exogenous hyaluronic acid (HA) could reduce the infectivity of R5-HIV. Unstimulated CD4 + T cells were treated with hyaluronidase to study the effect of endogenous HA on R5-HIV infectivity. Finally, the effect of exogenous and endogenous hyaluronic acid (HA) on the adhesion of R5-HIV to CD4 + T cells was also measured. Results (1) 100μg exogenous HA treatment could significantly reduce R5-HIV-infected TZM-bl cells and unstimulated CD4 + T cells (P <0.001). (2) Hyaluronidase treatment can enhance the infectivity of HIV, but hyaluronidase treatment can be reversed (P <0.001) if 100 μg exogenous HA is added. (3) Exogenous HA reduced R5-HIV adhesion to CD4 + T cells, and hyaluronidase increased R5-HIV adhesion to CD4 + T cells (P <0.001). Conclusions Exogenous HA reduces the infectivity of unstimulated CD4 + T cells by R5-HIV, whereas hyaluronidase enhances the binding of R5-HIV to CD4 + T cells and enhances the effect of R5-HIV on CD4 + T cell infectivity.