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目的通过分析溃疡性结肠炎(UC)患者肠黏膜中CD4+CD2+5调节性T细胞及其特异性标志物Foxp3的表达特点,探讨CD4+CD2+5调节性T细胞和Foxp3在UC发病机制中的作用。方法 18例UC活动期患者和12例健康体检者作为正常对照组,分别经电子肠镜活检肠黏膜组织,以流式细胞术检测肠固有层单个核细胞(LPMC)中CD4+CD2+5T细胞和CD4+T细胞比例,应用免疫组化方法检测肠黏膜中Foxp3的表达。结果 UC患者肠黏膜CD4+CD2+5T细胞占CD4+T细胞比例和Foxp3+T细胞表达明显高于正常对照组(P<0.05),两组LPMC中CD4+T细胞比例无明显差异(P>0.05)。结论 CD4+CD2+5调节性T细胞在UC的发病机制中起重要作用,参与肠黏膜炎症的发生、发展,Foxp3是CD4+CD2+5调节性T细胞的特异性表达因子,外周血调节性T细胞数量减少可能是疾病发生的重要因素。
Objective To investigate the expression of CD4 + CD2 + 5 regulatory T cells and its specific marker Foxp3 in the intestinal mucosa of patients with ulcerative colitis (UC) and to explore the role of CD4 + CD2 + 5 regulatory T cells and Foxp3 in the pathogenesis of UC In the role. Methods Eighteen patients with active UC and 12 healthy controls were enrolled as normal control group. The intestinal mucosa were harvested by electron microscopy. Flow cytometry was used to detect CD4 + CD25 + T cells in lamina propria mononuclear cells (LPMC) And CD4 + T cell ratio, the expression of Foxp3 in intestinal mucosa was detected by immunohistochemistry. Results The percentage of CD4 + CD25 + T cells in CD4 + T cells and Foxp3 + T cells in UC patients were significantly higher than those in normal controls (P <0.05). There was no significant difference in the proportion of CD4 + T cells between two groups (P> 0.05). Conclusions CD4 + CD25 + regulatory T cells play an important role in the pathogenesis of UC and are involved in the occurrence and development of intestinal mucosal inflammation. Foxp3 is a specific expression of CD4 + CD25 + regulatory T cells and peripheral blood regulatory Decreasing T cell numbers may be an important factor in disease development.