制备特异性抗体在分子生物学研究及病毒诊断学研究中起重要作用。以往多采用抗原免疫家兔后采集兔血清制备多克隆抗体,或采用杂交瘤技术在 Balb/C 小鼠中制备单克隆抗体腹水,我们利用 S180腹水瘤在小鼠中制备多克隆抗体。以重组 Toscana 病毒 N 蛋白为例:初次免疫用20μg重组蛋白与等体积福氏完全佐剂混合,腹腔注射普通 Swiss 小鼠,第2周和第4周用同样剂量蛋白与等体积不完全福氏佐剂混合腹腔免疫,第5周,眼球取 Preparation of specific antibodies in molecular biology and viral diagnostic studies play an important role. In the past, rabbits were immunized with antigens to collect rabbit serum to prepare polyclonal antibody, or hybridoma technique was used to prepare monoclonal antibody ascites in Balb / C mice. We made polyclonal antibodies in mice using S180 ascites tumor. The recombinant Toscana virus N protein as an example: the initial immunization with 20μg recombinant protein and complete Freund’s adjuvant mixed with normal intraperitoneal injection of ordinary Swiss mice, the first two weeks and four weeks with the same dose of protein and incomplete volume of Freudenberg Adjuvant mixed intraperitoneal immunization, the first 5 weeks, eyeball taken