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为了准确快速地评价香蕉对枯萎病病原菌尖孢镰刀菌古巴专化型(Fusarium oxysporum f.sp.cubense)热带4号小种(Tropical race 4,Foc TR4)的抗性,在温室条件下以浸根法和改良的灌根法将Foc TR4接种至不同抗性的香蕉品种,比较分析接种后香蕉的发病情况,并检测了两个Foc TR4的特异引物的扩增效率,以完善评价方法。结果显示,利用两种方法接种都能体现香蕉的抗性,但改良的灌根法在孢子浓度为1×10~4时的接种效果最好,接种后各品种发病情况与品种本身的实际抗性最为接近。特异引物Foc TR4 F/R的扩增效率较高,1次和2次PCR分别能有效检测发病等级为3和1以上的香蕉球茎。
In order to accurately and rapidly evaluate the resistance of banana to the Fusarium oxysporum f.sp.cubense Tropical race 4 (Foc TR4) Foc TR4 was inoculated into banana cultivars with different resistances by rooting method and improved irrigation rooting method. The incidence of banana after inoculation was compared and the amplification efficiency of two Foc TR4 specific primers was tested to improve the evaluation method. The results showed that the two methods of inoculation can reflect the resistance of bananas, but the improved method of irrigation under the spore concentration of 1 × 10 ~ 4 when the inoculation effect of the best after inoculation, the incidence of each species and the actual species itself The closest sex. The specific primers Foc TR4 F / R amplification efficiency is higher, 1 and 2 PCR were effective detection of incidence levels of 3 and 1 banana bulbs.