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目的 通过在脊髓神经元培养中应用X线照射 ,选择最佳时机 ,从而抑制或杀伤非神经元细胞增殖 ,提高神经元存活率 ,促进其生长、分化。方法 对E18大鼠胚胎脊髓神经元培养 ,在培养当天 ,第 1、2、3、4天行X线照射 ,剂量为 2Gy。在培养 2周时 ,采用链霉素抗生素蛋白 过氧化酶连接法 (SP)免疫组织化学方法显示神经元特异性烯醇化酶 (NSE)作为判定照射效果的依据。结果 在培养第 2天时给予 2Gy的X线照射 ,NSE阳性细胞数最高 ,为 (3 5 3± 42 )个 ,第 2天照射组明显多于其他组 (P <0 .0 1)。结论 在培养第 2天时给予 2Gy的X线照射 ,大鼠胚胎脊髓神经元存活细胞数明显高于其他培养时间。
OBJECTIVE: To select the optimal timing by applying X-ray irradiation in spinal cord neuron culture, so as to inhibit or kill the proliferation of non-neuronal cells, enhance the survival rate of neurons, and promote their growth and differentiation. Methods The embryonic spinal cord neurons of E18 rats were cultured on day 1, 2, 3, and 4 with the dose of 2Gy. At 2 weeks of culture, neuron-specific enolase (NSE) was used as a basis for determining the effect of irradiation using streptavidin-peroxidase-linked immunosorbent assay (SP). Results The 2Gy X-ray irradiation on the second day of culture showed the highest number of NSE positive cells (353 ± 42), the second day was significantly more than the other groups (P <0.01). Conclusion The number of surviving neurons in embryonic spinal cord neurons was significantly higher than that in other culture days when irradiated with 2Gy radiation for 2 days.