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目的:观察金雀异黄酮对缺氧成骨细胞的抗缺氧作用。方法:以大鼠成骨细胞为材料,用三气培养箱建立缺氧模型。设常氧对照组、缺氧对照组和含不同浓度金雀异黄酮的缺氧加药组。比较各组缺氧36 h后细胞活力、ROS含量、细胞凋亡、细胞周期、PCNA表达i、NOS活性和钙化结节面积等,荧光定量PCR检测HIF-1α、BCL-2及Caspase-3 mRNA的表达。结果:金雀异黄酮显著提高缺氧成骨细胞存活率、G1期细胞百分比、钙化结节面积、HIF-1α和BCL-2 mRNA的表达水平,降低凋亡、ROS含量、PCNA表达量i、NOS酶活性和Caspase-3 mRNA表达。结论:金雀异黄酮能保护缺氧成骨细胞并促进其分化。
Objective: To observe the anti-hypoxia effect of genistein on hypoxia osteoblasts. Methods: Rat osteoblasts were used as materials to establish hypoxia model with three-gas incubator. The normoxia control group, hypoxia control group and hypoxia group with different concentrations of genistein were added. Cell viability, ROS content, cell apoptosis, cell cycle, PCNA expression i, NOS activity and calcified nodule area were compared after 36 h hypoxia. HIF-1α, BCL-2 and Caspase-3 mRNA expression. Results: Genistein significantly increased the survival rate of hypoxic osteoblasts, the percentage of cells in G1 phase, the area of calcified nodules, the expression of HIF-1α and BCL-2 mRNA, the decrease of apoptosis, the content of ROS, the expression of PCNA, NOS enzyme activity and Caspase-3 mRNA expression. Conclusion: Genistein can protect hypoxia osteoblasts and promote their differentiation.