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目的研究2015年成都市流感流行情况,并分析监测影响因素。方法收集哨点医院监测的ILI咽拭子,进行流感病毒的病毒培养分离和病毒核酸检测。结果流感病毒的阳性率为16.57%,其中甲型H1N1、H3N2、B-Vic、BYam,阳性率分别为0.065%、11.45%、0.13%、4.74%;还有0.19%的混合感染,H1+H3、H3+BY和BV+BY分别为0.032%、0.13%、0.032%。核酸检测法的灵敏度和特异性均高于病毒分离法。标本的合格率影响病毒分离的结果。讨论 2015年以H3N2和B-Yam交替为流感优势毒株,并有混合感染的存在,为流感的重组变异提供了条件。采用两种方法检测流感病毒,并提高标本质量,获取准确的监测资料,以指导流感的防控。
Objective To study the prevalence of influenza in Chengdu in 2015 and analyze the influencing factors. Methods ILI pharyngeal swabs collected from sentinel hospitals were collected for virus isolation and virus nucleic acid detection of influenza virus. Results The positive rate of influenza virus was 16.57%. The positive rates of influenza A H1N1, H3N2, B-Vic and BYam were 0.065%, 11.45%, 0.13% and 4.74%, respectively. There was also 0.19% mixed infection and H1 + H3 , H3 + BY and BV + BY were 0.032%, 0.13% and 0.032%, respectively. The sensitivity and specificity of the nucleic acid detection method are higher than the virus isolation method. The passing rate of specimens affects the result of virus isolation. Discussion In 2015, H3N2 and B-Yam were alternately dominant strains of influenza with mixed infection, which provided the conditions for the recombinant variation of influenza. Two methods are used to detect influenza virus, improve the quality of specimens and obtain accurate monitoring data to guide the prevention and control of influenza.