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目的:探讨SOD对青石棉诱导BEAS-2B细胞产生过氧化物歧化酶(ROS)的抑制作用。方法:采用台盼蓝吞噬实验和乳酸脱氢酶同工酶(LDH)试剂盒分别测定0mg/L、100mg/L、250mg/L、500mg/L青石棉作用后BE-AS-2B细胞成活率和培养上清LDH水平。分别采用双氢溴乙啶(DHE)和双氯荧光素双醋酸盐(DCF)荧光探针测定青石棉刺激BEAS-2B细胞后ROS的产生情况,同时使用200USOD与青石棉共孵育观察其对ROS产生的抑制作用。结果:100mg/L以下剂量的青石棉与BEAS-2B细胞共培养24h后细胞成活率为97%,未显示明显毒性;DHE荧光染色显示青石棉可诱导BEAS-2B细胞产生ROS,细胞呈深红染色,使用SOD后可抑制ROS产生,细胞染色减弱;且不同剂量青石棉作用BEAS-2B细胞不同时间后DCF染色差异无统计学意义(P>0.05)。结论:青石棉可诱导BEAS-2B细胞产生ROS,且该作用可被SOD抑制。
Objective: To investigate the inhibitory effect of SOD on catalase (ROS) induced by crocidolite in BEAS-2B cells. Methods: The survival rate of BE-AS-2B cells was determined by trypan blue engorgement test and lactate dehydrogenase isoenzyme (LDH) kit after being treated with 0, 100 mg / L, 250 mg / L and 500 mg / L crocidolite And culture supernatant LDH level. The production of ROS induced by crocidolite on BEAS-2B cells was detected by DHE and DCF fluorescent probes respectively. Meanwhile, 200USOD and crocidolite Inhibition of ROS production. Results: The survival rate of BEAS-2B cells incubated with crocidolite at a dose of 100 mg / L for 24 h was 97%, showing no obvious toxicity. DHE staining showed that crocidolite could induce ROS production in BEAS-2B cells and dark red Staining, the use of SOD can inhibit ROS production, cell staining weakened; and different doses of crocidolite BEAS-2B cells after different time DCF staining was no significant difference (P> 0.05). CONCLUSION: Crocidolite can induce ROS production in BEAS-2B cells and this effect can be inhibited by SOD.