【摘 要】
：
为在临床诊断中快速鉴定副猪嗜血杆菌(HPS)血清4型和13型等优势流行血清型,分子血清分型方法较常规的基于特异性抗血清的血清分型方法更加准确和高效。根据HPS血清4型脂多糖
【机 构】
：
北京市农林科学院畜牧兽医研究所/畜禽疫病防控技术北京市重点实验室
【基金项目】
：
国家重点研发计划项目(2016YFD0500706),国家自然科学基金项目(31572527),北京市农林科学院科技创新能力建设专项项目(KJCX201914)
论文部分内容阅读
为在临床诊断中快速鉴定副猪嗜血杆菌(HPS)血清4型和13型等优势流行血清型,分子血清分型方法较常规的基于特异性抗血清的血清分型方法更加准确和高效。根据HPS血清4型脂多糖合成蛋白基因wcip 4和血清13型糖脂转移蛋白基因gltP序列设计特异性引物,分别建立了简便快速鉴定HPS血清4型和13型的环介导等温扩增方法(LAMP)。特异性检测结果显示,HPS血清4型或13型参考菌株检测为阳性,而其他HPS血清型参考菌株及19种其他细菌检测均为阴性;敏感性检测结果显示,在25μL反应体系中,HPS血清4型和13
其他文献
分析激光加工项目融合于传统工程训练教学中的可行性,以激光加工课程为基础,阐述激光加工如何以“翻转课堂”和“固定学时+实验室开放”的教学模式进行教学内容设计,探索如何
摩根模型(Mokken model)是项目反应理论模型中的非参数模型。摩根模型不仅可以处理二值计分的项目,也可以处理多值计分的项目。摩根模型以同质系数H和Z分数作为判定标准,H值
The development of new kinds of semiconductor material is a very attractive topic of scientific research and appliations.Graphene is considered as one of the mo
Silicon-vacancy(VSi)centers in silicon carbide(SiC)are expected to serve as solid qubits,which can be used in quantum computing and sensing.As a new controllabl
Graphene has been extensively explored to enhance functional and mechanical properties of metalmatrix nanocomposites for wide-range applications due to their su
As a promisingmaterial for quantumtechnology,silicon carbide(SiC)has attracted great interest inmaterials science.Carbon vacancy is a dominant defect in 4H-SiC.
Analysis of the short-circuit characteristics of SiC metal-oxide-semiconductor field-effect transistors(MOSFETs)is very important for their practical applicatio
Commercially available AlGaN/GaN high-electron-mobility transistors(HEMTs)are beginning to enter the public scene froma range of suppliers.Based on previous stu
Although a high-quality homoepitaxial layer of 4H‑silicon carbide(4H-SiC)can be obtained on a 4°off-axis substrate using chemical vapor deposition,the red