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目的探讨慢性乙型肝炎患者血清中Th1/Th2型细胞因子水平与病情轻重、活动程度的关系。方法采用双抗体夹心ELISA法检测73例不同病情程度、不同ALT水平慢性乙型肝炎患者血清中Th1型细胞因子和Th2型细胞因子各3种。结果慢性乙型肝炎患者血清中3种Th1型细胞因子IL_2、IFN_γ、IL_12水平普遍较低,各组间差异多无统计学意义。3种Th2型细胞因子IL_4、IL_6、IL_10的各组间差异均无统计学意义,其中IL_6水平普遍较高,慢性重型肝炎组和乙型肝炎肝硬化组患者血清IL_4和IL_10水平相对较高。6种细胞因子水平与患者ALT值之间均呈正相关。结论慢性乙型肝炎患者血清中Th1型细胞因子IL_2、IFN_γ、IL_12和Th2型细胞因子IL_4、IL_6、IL_10水平可反映其病情活动程度更甚于病情轻重程度。
Objective To investigate the relationship between serum Th1 / Th2 cytokines and severity of illness and activity in patients with chronic hepatitis B Methods Serum levels of Th1 cytokines and Th2 cytokines in 73 patients with different severity of ALT were detected by double antibody sandwich ELISA. Results The levels of three kinds of Th1 cytokines, IL_2, IFN_γ and IL_12 in serum of patients with chronic hepatitis B were generally low, with no significant difference among the groups. The levels of IL-4, IL-6 and IL-10 among the three kinds of Th2 cytokines were not significantly different among all groups. IL-6 level was generally higher in patients with chronic severe hepatitis and cirrhosis than in patients with chronic hepatitis B and IL-4. Six kinds of cytokines levels and patients with ALT values were positively correlated. Conclusion The levels of Th1 cytokines IL_2, IFN_γ, IL_12 and Th2 cytokines IL_4, IL_6 and IL_10 in serum of patients with chronic hepatitis B can reflect the degree of disease activity more than the severity of disease.