论文部分内容阅读
本研究为筛选在岩白菜中稳定表达的内参基因,以用于岩白菜实时荧光定量PCR研究,以岩白菜的幼叶、成熟叶、叶柄、根、一年生根状茎和二年生根状茎为材料,采用实时荧光定量PCR技术比较了GAPDH、18S rRNA、β-actin、TUA、UBQ5和TUB等6个候选内参基因的表达情况,并经Ge Norm、Norm Finder和Best Keeper等3种软件分析,分析表明6个候选内参基因在岩白菜不同器官中的表达情况存在差异,其中GAPDH和18S rRNA的表达都较为稳定,可作为岩白菜实时荧光定量PCR分析的内参基因。
In this study, we screened the reference genes stably expressed in Brassica campestris for the real-time quantitative PCR study of Brassica campestris. With young leaves, mature leaves, petioles, roots, annual rootstocks and biennial rhizomes as material The expression of 6 candidate internal reference genes, GAPDH, 18S rRNA, β-actin, TUA, UBQ5 and TUB, was compared by real-time fluorescence quantitative PCR and analyzed by Ge Norm, Norm Finder and Best Keeper software The results showed that there were differences in the expression of six candidate reference genes in different organs of Chinese cabbage. The expression of GAPDH and 18S rRNA were stable and could be used as reference genes for real-time quantitative PCR analysis of Brassica campestris.