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为探讨抑癌基因 CDKN2和癌基因 ras 与喉癌临床特征的关系,分别采用免疫组织化学染色 SP 法和双抗体标记流式细胞法,检测57例喉癌及正常喉粘膜上皮细胞内 CDKN2基因产物 P16蛋白和 ras 基因产物 P21蛋白的表达。结果显示,正常喉粘膜上皮细胞内 P16阳性率(95%)显著高于喉癌细胞(45.6%),P<0.01;P16阳性率在喉癌Ⅰ期(66.7%)和Ⅱ期(57.1%),都分别显著高于喉癌Ⅲ期(37.5%)和Ⅳ期(38.9%),P<0.05;颈淋巴结转移组 P16蛋白阳性率(36.4%)显著低于非转移组(47.8%);正常喉粘膜上皮细胞内 P21蛋白为阴性,喉癌细胞内 P21蛋白的阳性率为78.9%;高分化鳞癌组 P21蛋白的阳性率(89.5%)显著高于低分化组(70%),P<0.05。结论:抑癌基因 CDKN2的失活、丢失和癌基因 ras 的激活参与了喉癌的发生;P16蛋白的表达缺失、P21蛋白的阳性表达是反映喉癌细胞生物学行为及临床特征的较好指标。
To investigate the relationship between the tumor suppressor gene CDKN2 and the oncogene ras and the clinical features of laryngeal carcinoma, the expressions of CDKN2 gene in 57 laryngeal carcinomas and normal laryngeal mucosal epithelial cells were detected by immunohistochemical SP method and double antibody labeled flow cytometry P16 protein and ras gene product P21 protein expression. The positive rate of P16 in normal laryngeal mucosa epithelial cells was significantly higher than that in laryngeal carcinoma cells (45.6%, P <0.01). The positive rate of P16 in laryngeal carcinoma stage Ⅰ (66.7%) and stage Ⅱ (57.1% , Respectively (P <0.05). The positive rate of P16 protein in cervical lymph node metastasis group was significantly lower than that in non-metastatic group (47.8%) The positive rate of P21 protein in laryngeal carcinoma cells was 78.9%. The positive rate of P21 protein in well differentiated squamous cell carcinoma was significantly higher than that in poorly differentiated squamous cell carcinoma (P < 0.05. CONCLUSION: The inactivation and loss of the tumor suppressor gene CDKN2 and the activation of the oncogene ras are involved in the carcinogenesis of laryngeal carcinoma. The expression of P16 protein is absent and the positive expression of P21 protein is a good indicator to reflect the biological behavior and clinical features of laryngeal cancer cells .