论文部分内容阅读
目的 用新西兰兔建立糖尿病性勃起功能障碍(ED)动物模型,为ED的基因治疗提供实验基础。方法 雄性新西兰白兔(2.4~2.6)kg30只,实验兔15只采用四氧嘧啶耳缘静脉注射(150mg/kg)诱发糖尿病,对照组兔15只耳缘静脉注射等量生理盐水。在相同条件下饲养,6月后比较试验组兔和对照组兔阴茎勃起功能的改变:方法是测量两组兔基础海绵体内压(ICP)和电刺激盆神经后检测ICP的变化,同时检测颈动脉血压(SAP),鉴定ED兔的建立。结果 用四氧嘧啶诱导的糖尿病兔的血糖值明显高于对照组兔,血糖均大于20mmol/L,体重增加小于对照组兔,两组数值有显著性差异。两组兔基础ICP无明显差异,P>0.05;电刺激盆神经时,糖尿病组兔的平均ICP为(11.651.40)mmHg;而对照组兔为(33.613.20)mmHg,P<0.01,差异有显著性。结论 用四氧嘧啶可诱导新西兰雄兔建立糖尿病动物模型,进一步建立ED的动物模型,为研究ED的基因治疗奠定实验基础。
Objective To establish an animal model of diabetic erectile dysfunction (ED) with New Zealand rabbits to provide experimental basis for gene therapy of ED. Methods Thirty male New Zealand white rabbits (2.4-2.6 kg) were randomly divided into experimental group (n = 15) and experimental group (n = 15). All rabbits were induced with diabetes by intravenous injection of 150 mg / kg alloxan. After 6 months, the penile erectile function of rabbits in control group and control group were compared. The ICP method was used to measure the changes of intracapsular pressure (ICP) Arterial blood pressure (SAP) was used to identify the establishment of ED rabbits. Results Alloxan induced diabetes rabbits blood glucose was significantly higher than the control group, blood glucose were greater than 20mmol / L, body weight gain was less than the control group, the two groups had significant differences. The ICP of the rabbits in the two groups had no significant difference (P> 0.05). The average ICP of rabbits in the diabetic group was (11.651.40) mmHg while the control group was (33.613.20) mmHg, P <0.01 Significant. Conclusion Alloxan can induce New Zealand male rabbits to establish animal model of diabetes and further establish ED animal model for the study of ED gene therapy lay the experimental foundation.