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目的探讨侧脑室注射N-甲基-D-天冬氨酸受体2B(NR2B)拮抗剂Ro25-6981对大鼠脑局灶性缺血-再灌注(I-R)损伤的影响。方法60只雄性SD大鼠随机均分为四组:假手术组(S组),I-R组,I-R+Ro25-6981组(I-R+Ro组)和I-R+生理盐水组(I-R+NS组)。S组大鼠线栓置入颈内动脉的深度为10mm;I-R组大鼠采用线栓法阻闭大脑中动脉,2h后拔出线栓到颈外动脉给予再灌注;I-R+Ro组和I-R+NS组于缺血后2h脑室内分别注射Ro25-6981 0.1mol/L和生理盐水各5μl。于再灌注24h后行神经功能缺损评分;麻醉大鼠取脑组织,切片后用2,3,5-氯化三苯基四氮唑染色,Image Pro Plus 6.0图像处理软件计算大鼠脑梗死容积。结果与S组相比,I-R组大鼠神经功能缺陷评分增加,脑梗死容积增大(P<0.05或P<0.01);与I-R组相比,I-R+Ro组大鼠神经功能缺损评分降低,脑梗死容积减小(P<0.05或P<0.01)。结论大鼠缺血2h后脑室内注射NR2B拮抗剂Ro25-6981可以降低神经功能缺损评分、缩小脑梗死容积,对大鼠局灶性脑I-R损伤有一定保护作用。
Objective To investigate the effects of intracerebroventricular injection of Ro25-6981, an N-methyl-D-aspartate receptor 2B (NR2B) antagonist, on focal brain ischemia-reperfusion (I-R) injury in rats. Methods Sixty male Sprague-Dawley rats were randomly divided into four groups: sham operation group (S group), IR group, I-R + Ro25-6981 group (I-R + Ro group) and I-R + -R + NS group). The rats in group S received a depth of 10 mm into the internal carotid artery. The rats in group IR blocked the middle cerebral artery by thread occlusion. The rats in group IR were withdrawn after 2 hours and re-injected into the external carotid artery. And I-R + NS group were intracerebroventricular injection of Ro25-6981 0.1mol / L 2h after ischemia and saline 5μl. Neurological impairment scores were performed 24h after reperfusion. Brain tissue was taken from anesthetized rats and stained with 2,3,5,5-triphenyltetrazolium chloride and image Pro Plus 6.0 image processing software to calculate the cerebral infarction volume . Results Compared with group S, IR score increased and cerebral infarction volume increased in IR group (P <0.05 or P <0.01). Compared with IR group, neurological deficit score Decreased, cerebral infarction volume decreased (P <0.05 or P <0.01). Conclusion Intraventricular injection of Ro25-6981, an NR2B antagonist, can decrease the neurological deficit score and decrease the volume of cerebral infarction 2 h after ischemia in rats, which may have a protective effect on the focal cerebral I-R injury in rats.