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目的 研究BMP 2是否对胶质瘤细胞也具有诱导凋亡作用。方法 培养的人脑胶质瘤细胞系SHG 4 4中加入rhBMP 2 ,运用透射电镜观察凋亡的超微结构 ,流式细胞仪技术分析细胞DNA周期变化 ,琼脂糖凝胶电泳检测DNA片段的改变。结果 电镜下可见细胞核染色质浓缩、边集、核碎裂 ;DNA周期分析显示在G1期峰前存在一个凋亡峰 ( 12 1% ) ;DNA电泳呈梯状。结论 rhBMP 2在体外对人脑胶质瘤细胞系SHG 4 4有明显诱导凋亡的作用。
Objective To investigate whether BMP 2 also induces apoptosis in glioma cells. Methods The human glioma cell line SHG 4 4 was cultured with rhBMP 2. The ultrastructure of apoptotic cells was observed by transmission electron microscopy. The changes of DNA cycle were analyzed by flow cytometry. The DNA fragments were detected by agarose gel electrophoresis . Results Electron microscope showed chromatin condensation, margins and nuclear fragmentation. DNA cycle analysis showed that there was a peak of apoptosis (12 1%) before G1 peak. The DNA electrophoresis showed a ladder shape. Conclusion rhBMP 2 can induce apoptosis of human glioma cell line SHG 4 4 in vitro.