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目的:探讨低聚糖阿魏酸酯(CFOs)在由链脲佐菌素诱导的1型糖尿病怀孕大鼠体内的降血糖和抗氧化作用。方法:取40只大鼠做实验,共分五组(每组8只):(1)未交配过的糖尿病鼠为对照组;(2)交配过的非糖尿病鼠为对照组;(3)交配过的糖尿病鼠为对照组;(4)未交配过的糖尿病鼠为治疗组;(5)交配过的糖尿病鼠为治疗组。治疗组在大鼠交配前3 d开始,连续15 d给大鼠喂饲玉米低聚糖阿魏酸酯植物提取物(25 mg/kg)。对照组动物则只接受溶媒处理(2.5 mL/kg)。在交配后的第14天,处死大鼠,从下腔静脉采集母体血液,做ELISA检测。交配过的大鼠应解剖其子宫以搜寻任何可能存在的胎儿。每只大鼠的肝脏都被迅速切除,用Jamall和Smith的检测方法来确定组织样本中脂质过氧化物的水平。结果:不考虑怀孕因素,低聚糖阿魏酸酯的使用可降低血糖值(P<0.05)。使用低聚糖阿魏酸酯治疗未孕和怀孕大鼠后,两者血清胰岛素水平均上升(P<0.05)。使用低聚糖阿魏酸酯并未对血浆内的硫代巴比妥酸反应物水平产生显著影响(P>0.05)。实验动物被诱导患糖尿病后,再用低聚糖阿魏酸酯进行治疗,其谷胱甘肽水平的平均值有所下降(P<0.05)。糖尿病孕鼠治疗组和孕鼠对照组肝脏内的硫代巴比妥酸反应物水平和谷胱甘肽水平,差异有统计学意义(P<0.05)。结论:低聚糖阿魏酸酯可降低怀孕及未怀孕糖尿病大鼠体内的血糖浓度、提高血清胰岛素浓度;与肝脏有关的脂质过氧化反应上升和谷胱甘肽的下降发生逆转。
Objective: To investigate the hypoglycemic and anti-oxidative effects of oligo-ferulate (CFOs) in type 1 diabetic pregnant rats induced by streptozotocin (STZ). Methods: Forty rats were divided into five groups (n = 8): (1) untreated diabetic rats as control group; (2) non-diabetic mice as control group; (3) Diabetic mice were used as control group; (4) Unpaired diabetic rats were treated group; (5) Diabetic mice were treated group. The rats in the treatment group were fed with maize oligosaccharides ferulic acid ester extract (25 mg / kg) on the 3rd day before mating and 15 days after the rats were treated. Control animals received only vehicle (2.5 mL / kg). On the 14th day after mating, rats were sacrificed and maternal blood was collected from the inferior vena cava for ELISA testing. Mating rats should dissect their uterus to search for any possible fetus. The liver of each rat was rapidly excised and the levels of lipid peroxides in the tissue samples were determined using the detection method of Jamall and Smith. Results: Regardless of pregnancy, the use of oligosaccharides ferulate decreased blood glucose (P <0.05). After treatment with oligo-ferulate for both un-pregnant and pregnant rats, serum insulin levels increased (P <0.05). The use of oligosaccharides ferulic acid ester did not significantly affect the level of thiobarbituric acid in plasma (P> 0.05). After the animals were induced to develop diabetes mellitus, they were treated with oligosaccharides ferulic acid esters, and the mean level of glutathione decreased (P <0.05). The levels of thiobarbituric acid reactant and glutathione in the liver of the pregnant mice and the control group were significantly different (P <0.05). CONCLUSION: Oligosaccharides ferulic acid ester can reduce the blood glucose level in pregnant and non-pregnant diabetic rats and increase the serum insulin concentration. The liver-related lipid peroxidation reaction and glutathione decline are reversed.