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抗体介导的纤溶剂是以抗体与血栓呈高选择的结合为基础的。本研究利用自制的抗人活化血小板α-颗粒膜蛋白(GMP—140)的单抗SZ—51,经木瓜蛋白酶消化制备抗体的Fab片段,与纤溶剂尿激酶的B链经化学双功能交联剂SPDP交联后,其嵌合分子的体外溶栓率较尿激酶高3～5倍,而血浆中纤维蛋白原及纤溶酶原的消耗减少,提示溶栓率的提高与嵌合分子的选择性增高有关,这种新型的嵌合分子将为体内特异性溶栓剂的研制提供一条新的途径。
Antibody-mediated fibrinolysis is based on the combination of high antibody and thrombus selectivity. In this study, a Fab fragment of the antibody was prepared by papain digestion of a homemade monoclonal antibody SZ-51 against human activated platelet alpha-granule membrane protein (GMP-140) and chemically bifunctional crosslinked with the fibronectin B chain After SPDP cross-linking, the in vitro thrombolysis rate of its chimeric molecules is 3 to 5 times higher than that of urokinase, while the consumption of fibrinogen and plasminogen in plasma is reduced, suggesting that the improvement of thrombolysis rate is associated with the increase of chimeric molecule Selective increase, this new chimeric molecule will provide a new way for the development of in vivo specific thrombolytic agents.