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目的 了解冻干乙脑活疫苗在不同温度下保存对滴度及免疫力的影响。方法 冻干乙脑活疫苗 在不同温度下存放不同时间后，进行病毒滴度及免疫力检测。结果 疫苗在４－８％下存放１年半滴度稍有下降 （０．３２ＬｏｇＰＦＵ），其免疫力仍稳定在高水平（ＩＤ５０＝４．１×１０－５；在 ２５℃下存放 １个月和 ３７℃下存放７ｄ，滴度分别下降 ０．３２和 ０．６５ＬｏｇＰＦＵ，其免疫力仍保持在较高范围内（ＩＤ＝８．８ ×１０－5和 ６．０×１０－５）；在５０℃下存放２４ｈ滴度和免疫 力均显著下降（近１０倍）；存放７２ｈ后基本无免疫力。结论 疫苗不论在４－８、２５、３７℃下存放，其滴度保持在４． ８７ＬｏｇＰＦＵ以上时，仍有免疫力；疫苗滴度不低于５．７ＬｏｇＰＦＵ／ｍｌ时，仍保持高免疫力。
Objective To understand the effect of freeze-dried JE live vaccine on titer and immunity at different temperatures. Methods Lyophilized JE live vaccine was stored at different temperatures for different time, and the virus titer and immunity were tested. Results The vaccine showed a slight decrease of 1 year and a half titers (0.32 LogPFU) when stored at 4-8% and its immunity remained stable at a high level (ID50 = 4.1 × 10-5; stored at 25 ° C for 1 month And kept at 37 ℃ for 7 days, the titers decreased by 0.32 and 0.65LogPFU, respectively, and their immunity still remained in the higher range (ID = 8.8 × 10-5 and 6.0 × 10-5) The titer and immunity were significantly decreased (nearly 10-fold) when stored at 50 ℃ for 24 h and basically no immunity for 72 h after storage.Conclusion The vaccine maintained its titer no matter at 4-8,25 and 37 ℃, Above, there is still immunity; vaccine titer of not less than 5.7LogPFU / ml, still maintain high immunity.