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目的:建立独一味软胶囊中8-epideoxyloganic acid的含量测定方法。方法:采用高效液相色谱法。色谱柱:迪马C18(4.6 mm×250 mm,5μm),柱温40℃,流动相甲醇-0.025 mol·L-1磷酸水(50∶50),流速1.0 mL·min-1,检测波长238 nm。结果:8-epideoxyloganic acid进样量在1.979 2～9.896μg线性关系良好,回收率均在95.20%～100.00%,RSD均小于5%。结论:此方法准确可靠,重复性好,结果稳定,可作为独一味软胶囊及其他独一味制剂中环烯醚萜成分含量测定的方法。
Objective: To establish a method for the determination of 8-epideoxyloganic acid in Douchi soft capsules. Methods: Using high performance liquid chromatography. Column: Dima C18 (4.6 mm × 250 mm, 5μm), column temperature 40 ℃, mobile phase methanol-0.025 mol·L-1 phosphoric acid water 50:50, flow rate 1.0 mL · min-1, detection wavelength 238 nm. Results: The linearity of injection of 8-epideoxyloganic acid between 1.979 2 and 9.896 μg was good, and the recoveries were between 95.20% and 100.00% with RSD less than 5%. Conclusion: This method is accurate and reliable, good reproducibility and stable results. It can be used as a method to determine the content of iridoids in single-flavor soft capsule and other unique flavor preparations.