目前沿用的制作母种方法一般技术要求较高,为了简化制作母种手续,以便用最短的时间,最快的速度,生产出又多又好的母种。实践证明,只有采用优质母种才能生长出优质原种,进而生产出更多更好的栽培种。为此,我室于1986年春,大胆进行了“原种倒转管复壮法”制作母种,取得了较为理想的效果.(一)试验方法将组织分离法获得的试管斜面母种转瓶后的一级凤尾菇原种(培养基配方为棉籽壳78%,麸皮20%、熟石膏和白糖各1%),在接种箱内无菌操作,铲取黄豆粒大小的一块原种,放入装有PDA 培养基的试管斜面上,置恒温箱内在22℃下培养,3天后菌丝生长洁白、健壮,7天菌丝长 At present, the methods used for making mother species are generally higher in technical requirements. In order to simplify the procedures for producing mother species, in order to produce more and better mother species in the shortest time and at the fastest speed. Practice has proved that only the use of high-quality mother species can grow high-quality original species, and thus produce more and better cultivars. To this end, my room in the spring of 1986, boldly carried out “original species inversion tube rejuvenation law,” the production of mother species, and achieved a more satisfactory results. (A) test method Will be obtained by tissue isolation tube beveled mother species after bottle First grade Pleurotus ostreatus (medium recipe 78% cottonseed, bran 20%, plaster and white sugar each 1%), aseptic operation in the vaccination box, shovel a soybean seed size of a stock, put Into the PDA medium containing the tube inclined plane, set the incubator at 22 ℃ under culture, mycelial growth after 3 days white, robust, 7 days mycelial length