自从1975年Kohlev和Milstein证明骨髓瘤细胞与免疫动物脾细胞融合成杂交细胞系,并能分泌与免疫抗原相应抗体之后,它已成为许多领域中比较理想的工具。其应用范围也随着研究的深入,将越来越广。由于目前对细菌性疾病已有许多有力措施,细菌性传染病日趋减少,所以应用杂交瘤技术进行细菌学研究起步较迟,报道的文献较 Since 1975 Kohlev and Milstein have demonstrated that myeloma cells can fuse with immunized animal splenocytes into hybrid cell lines and can secrete antibodies that bind to immune antigens, making it an ideal tool in many fields. The scope of its application with the deepening of research will be more and more widely. Due to the many effective measures against bacterial diseases and the reduction of bacterial infectious diseases, the bacteriological study on the application of hybridoma started later. The reported literature