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目的:观察脂蛋白脂肪酶(lipoprotein lipas,LPL)在人胶质瘤U251细胞系中的表达及在leptomycin B(LMB)作用下对其增殖、生长的影响。方法:将经体外培养的U251细胞加入染色体区域稳定蛋白1(CRM1)抑制剂LMB后,分别于12 h和24 h观察细胞核与细胞质LPL表达情况,并利用MTT和流式细胞术检测细胞增殖和凋亡。结果:加入5、10 nmol/L的LMB孵育12 h和24 h后,细胞核内LPL的表达明显增加,且呈剂量依赖性变化,其中10 nmol/L的LMB孵育24 h,细胞核内LPL增高最明显;MTT显示,对照组和各实验组A值分别为(0.82±0.08)、(0.71±0.03)、(0.70±0.04)、(0.69±0.07)和(0.68±0.09),各实验组和对照组之间差异有统计学差异(P<0.05);流式细胞术检测显示各实验组与对照组U251细胞的凋亡率分别为2.81±0.33、4.17±0.24、5.25±0.31和6.80±0.42,各实验组与对照组间差异有统计学差异(P<0.05)。结论:LPL在U251细胞中的核质穿梭是依赖CRM1的,且LMB可以显著增加LPL在细胞核内的表达。随着LMB的浓度增加和孵育时间的延长,U251细胞的增殖和凋亡也受到显著的影响,提示核质穿梭可能对肿瘤的生长有一定的影响。
OBJECTIVE: To observe the expression of lipoprotein lipase (LPL) in human glioma U251 cell line and its effect on proliferation and growth of leptomycin B (LMB). Methods: The expression of LPL in nucleus and cytoplasm was observed at 12 h and 24 h after U251 cells cultured in vitro were added into LMB, an inhibitor of CRM1. MTT and flow cytometry were used to detect cell proliferation and Apoptosis. Results: The LPL expression in nuclei increased significantly and in a dose-dependent manner at 5 and 10 nmol / L LMB for 12 h and 24 h, respectively. Incubation with 10 nmol / L LMB for 24 h resulted in a significant increase in LPL (0.82 ± 0.08), (0.71 ± 0.03), (0.70 ± 0.04), (0.69 ± 0.07) and (0.68 ± 0.09) in the control group and each experimental group (P <0.05). The results of flow cytometry showed that the apoptosis rates of U251 cells in experimental group and control group were 2.81 ± 0.33, 4.17 ± 0.24, 5.25 ± 0.31 and 6.80 ± 0.42 respectively, The difference between the experimental group and the control group was statistically significant (P <0.05). CONCLUSION: LPL can activate the nucleoplasm in U251 cells and CRM-dependent LPL can significantly increase the expression of LPL in the nucleus. With the increase of LMB concentration and the extension of incubation time, the proliferation and apoptosis of U251 cells were also significantly affected, suggesting that the shuttle of cytoplasm may have a certain impact on tumor growth.