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为探讨米非司酮对人早孕期蜕膜组织细胞凋亡及相关基因Fas、FasL的影响 ,应用TUNEL法检测凋亡的发生 ,原位杂交和免疫组化法检测Fas、FasL的表达和相互关系。结果发现 :①正常早孕 4 0 + 天蜕膜组织细胞大量凋亡 ,Fas、FasLmRNA及蛋白有较强表达。②正常早孕 5 0 + 天 ,凋亡细胞明显减少 ,Fas、FasL的表达明显减弱。③早孕 5 0 + 天服用米非司酮 ,蜕膜组织大量凋亡 ,且Fas、FasL的表达较正常早孕 5 0 + 天增强。提示 ,米非司酮抗早孕的机制之一可能是通过促进蜕膜组织的异常凋亡实现的 ,Fas途径参与了这一过程
To investigate the effect of mifepristone on the apoptosis and related gene Fas, FasL in decidual tissue of early pregnancy, the apoptosis was detected by TUNEL method, the expression of Fas and FasL was detected by in situ hybridization and immunohistochemistry relationship. The results showed: (1) A large number of apoptotic cells were found in normal pregnant women on day 40, and Fas and FasL mRNA and protein were strongly expressed. ② On the 50th day of normal pregnancy, the number of apoptotic cells decreased significantly, and the expressions of Fas and FasL decreased significantly. ③ early pregnancy 50 + mifepristone, decidual tissue a large number of apoptosis, and Fas, FasL expression compared with normal early pregnancy 50 + day enhanced. Tip, mifepristone one of the mechanisms of anti-early pregnancy may be through the promotion of apoptosis of decidual tissue to achieve, Fas pathway involved in this process